Size : 0.5
P1(RMB) : 1318
MW : 1548.6
One letter sequence : Ac-DED(EDANS)-EE-Abu-psi-(COO)AS-K(DABCYL)-NH2
Molecular Formula : C68H89N15O25S
Description : A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
Literature Reference : Lin, C. et al. J. Biol. Chem. 10, 1074 (2004);
Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999);
Liu, Y. et al. Anal. Biochem. 267, 331 (1999);
Taliani, M. et al. Anal. Biochem. 240, 60 (1996).
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